I-Nucleic Acid Isizinda Noma Ikhithi Yokuhlanza
Nucleic Acid Eukukhishwa Or Ukuhlanzwa Kitnoma agcinwe ku -20 ℃. Isampula kufanele ithuthwe kusetshenziswa i-0℃ curling.
Introduction
I-Nucleic Acid Extraction Noma Ikhithi Yokuhlanza (Indlela Yobuhlalu Bezibuthe) yakhelwe ukuhlanzwa okuzenzakalelayo kwe-RNA ne-DNA oketshezini lomzimba (okufana nama-swabs, i-plasma, i-serum) kusetshenziswa izinsimbi zokukhipha i-nucleic acid ezizenzakalelayo. Ubuchwepheshe bezinhlayiya zikazibuthe buhlinzeka nge-DNA/RNA yekhwalithi ephezulu efanele ukusetshenziswa okuqondile ezinhlelweni ezisebenza ezansi nomfula ezifana nokukhulisa noma okunye ukusabela kwe-enzymatic.
Application Range
Igazi lonke, i-plasma, i-serum namanye amasampula ezicubu ahlanjululwa futhi agaywa. I-nucleic acid ekhishiwe yakhangiswa ngokukhetha ngobuhlalu obuzibuthe be-super paramagnetic nanometer. Khona-ke amaprotheni, ama-ion kasawoti we-inorganic kanye nokungcola okuphilayo kwasuswa ngokuwasha isisombululo.Ekugcineni, i-nucleic acid yakhishwa nge-eluent ukuthola isisombululo esihlanzekile se-nucleic acid.
Kit Contents
| Ikati. Cha. | I-YXN-VIRAL01-32A-BR | Izingxenye | ||
| -50A | - 100A | |||
| Usayizi | 32Tes | 50Isivivinyo | 100Test | |
| ULysis Buffer | 96 wellPre-pack ed Amapuleti 2 izingcezu | 25ml | 50ml | I-Surfactant ne-Tris |
| Wash Buffer I | ★15ml | ★30ml | Isixazululo esinosawoti omningi | |
| Geza i-Buffer Il | ★6ml*2 | ★12ml*2 | Isixazululo esinosawoti omncane | |
| I-Elution Buffer | 10ml | 20ml | Isixazululo esinosawoti omncane | |
| I-MagaBio Reagent | 1.0ml | 2.0ml | Izinhlayiya zikaZibuthe | |
| Ibhukwana(=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Nuthi:NgobaI-YXN-VIRAL01-32A-BR-50A,engeza u-15mL I-ethanol ephelele ku- ★15mL Wash Buffer I ngaphambi kokuyisebenzisa; engeza 24mL Absolute ethanol ukuze ★6mL Geza isigcina Il ngaphambi kokusebenzisa. | ||||
| NgobaI-YXN-VIRAL01-32A-BR-100A, engeza 30mL Absolute ethanol kokuthi ★30mL Wash Buffer I ngaphambi kokusebenzisa; engeza u-48mL I-ethanol ephelele kokuthi ★12mL Geza isilondolozi Il ngaphambi kokuyisebenzisa.【Izinto ezisetshenziswayo okufanele zilungiswe umsebenzisi】Sicela ulungise i-ethanol ephelele (ibanga lokuhlaziya) uwedwa. | ||||
Sti-orage Condkuyimions
Lapho kufika ikhithi, izingxenye zekhithi zingagcinwa ekamelweni lokushisa (15 - 25 ° C). Ama-reagents azinzile kuze kube unyaka owodwa kusukela ngosuku lokukhiqiza.
Sample Requirements
1. Isampula esebenzayo: I-Swabs, i-plasma, i-serum negazi eliphelele njll.
2. Ukugcinwa kwesampula kanye nokuthutha: Isampula kufanele ihlolwe ngokushesha
Materials futhi Devices Required but Not Provided
1. Amagilavu angenayo impushana alahlwayo
2. Isitsha se-Biohazard
3. Ipensela noma nge
ProCedure
Okulandelayo kusebenzisa isampula yokukhishwa kwe-swab lotion njengesibonelo ukuchaza kafushane izinyathelo zokusebenza ze-reagent ekhipha ithuluzi le-biological nucleic acid extraction.Bioer NPA-32PnomaSMUBUCIKO 32. Ukuze uthole ezinye izinhlobo zesampula, sicela ubheke kumanuwali yomsebenzisi. Ingase futhi isetshenziswe amakhasimende ngokuya ngokutholwa kokuhlola:
1. I-Reagent Ukulungiselela
a. NgobaI-YXN-VB03-32A-50A futhi I-YXN-VB03-32A-100A
Engeza 500uL Lysis Buffer kukholamu 1 kanye 7 ye-2.2mL 96-deep-well plate, 500uL Wash Buffer I kukholamu 2 kanye 8, 500uL Wash Buffer II kukholamu 3, 4 kanye 9,10; 70uL Elution Buffer kukholamu 5 kanye 11, 180uL amanzi ahlanzekile kanye 20uL MagaBio Reagent kuya kukholomu 6 kanye 12 (ubuhlalu kazibuthe kufanele buxutshwe kahle ngaphambi kokusetshenziswa),
b. NgobaI-YXN-VB03-32A
Beka ama-reagents apakishwe kahle angama-96 endaweni yezinga lokushisa. Nyakazisa ipuleti lemithombo engu-96 ulibhekise phansi izikhathi ezintathu, bese udabula isikhwama sepulasitiki. I-Centrifuge i-reagent epakishwe ngaphambilini imizuzwana embalwa (noma shwiba ngesandla izikhathi ezimbalwa) ukugwema ukunamathela odongeni lwamashubhu. Khipha ifilimu ye-aluminium foil yepuleti lemithombo engu-96 futhi uhlonze isiqondiso sepuleti (ubuhlalu kazibuthe kukholamu #6 kanye ne-#12),
2.Isampula Isizinda
1. Engeza isampula engu-300uL kumakholomu epuleti lomthombo angu-96 #1 kanye no-#7, sicela ugweme ukungcoliswa,
2. Beka ipuleti lomthombo elijulile elingu-96 ensimbini, faka amathiphu anemigqa engu-8 ensimbini,
3. Qalisa uhlelo ngokwezinqubo ezilandelayo,
4. Ngemva kokuphela kokuhlanza okuzenzakalelayo, dlulisela i-elution Buffer kumakholomu 5 no-11 kushubhu ye-anuclear engu-0.5mL ehlanzekile ye-centrifuge; uma ungayisebenzisi ngokushesha, sicela uyigcine ku-20 °C.
Performance Characteristics
1. Umkhiqizo okhishiwe utholwa nge-reagent yokutholwa kwe-HBV DNA ezwela kakhulu ukuze ufinyelele ukuzwela okungu-10 IU/mL. Umkhiqizo okhishiwe utholwa nge-reagent yokutholwa ye-HCV RNA ezwela kakhulu ukuze ufinyelele ukuzwela okungu-50 IU/mL.
2. Khetha amasampula e-4 (isampula ye-serum/plasma, isampula ye-nasopharyngeal swab, isampula yeseli ye-cervical exfoliated), isampula ngayinye ihlanjululwe izikhathi ezingu-10 ngama-gradients angu-3 (kuhlanganise nesamba sesampula sokuqala sokugxila kwe-4), kusetshenziswa ama-reagents afanelekayo kanye nama-ejenti okuhlola ukuze kutholwe ngaphakathi. isakhi sofuzo ngokwemiyalo yomkhiqizo, kanye nenani le-Ct leqoqo ngalinye lihluka ngaphansi kuka-1.
| Step | Hhayi-ke Indawo | Program Igama | Wakuyiming Time(min:SS) | Mixing Time(mku:SS) | Magnet Time(min:SS) | Ai-dsorption | Suchame | Volume ezintathu(μL) | Tumkhathi |
| 1 | 1 | Lysisi | 0:00 | 2:00 | 0:00 | F | 700 | 80 | |
| 2 | 6 | Bamadla | 0:00 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:00 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wumlotha1 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wumlotha2 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wumlotha3 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | I-Elution | 2:00 | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | Lahla | 0:00 | 0:15 | 0:00 | F | 200 |
Safety
1. UGENERAL UKUPHEPHA.
Ukusebenzisa lo mkhiqizo ngendlela engacacisiwe kumadokhumenti omsebenzisi kungase kubangele ukulimala komuntu siqu noma ukulimala kwethuluzi noma idivayisi. Qinisekisa ukuthi noma ubani osebenzisa lo mkhiqizo uthole imiyalelo ngendlela evamile yokuphepha kumalabhorethri kanye nolwazi lokuphepha olunikezwe kulo mbhalo.
1.1 Ngaphambi kokusebenzisa ithuluzi noma idivayisi, funda futhi uqonde imininingwane yokuphepha enikezwe kumadokhumenti omsebenzisi anikezwe umkhiqizi wethuluzi noma idivayisi.
1.2 Ngaphambi kokuphatha amakhemikhali, funda futhi uqonde wonke ama-Safety Data Sheets (SDS) asebenzayo futhi usebenzise izinto zokuzivikela ezifanele (amagilavu, izingubo zokugqoka, ukuvikela amehlo, njll.). Ukuze uthole ama-SDS, bheka ingxenye ethi “Imibhalo Nokusekela” kulo mbhalo.
2. Amakhemikhali ukuphepha
UKUPHATHA OKUJWAYELEKILE KWEKHEMICAL. Ukuze unciphise izingozi, qinisekisa ukuthi izisebenzi zaselabhorethri ziyafunda futhi zijwayelane neziqondiso ezijwayelekile zokuphepha zokusetshenziswa kwamakhemikhali, ukugcinwa, kanye nemfucuza enikezwe ngezansi, futhi uthintane ne-SDS efanele ukuze uthole izinyathelo zokuphepha ezithile neziqondiso: Funda futhi uqonde AmaSpredishithi Edatha Yezokuphepha (SDSs) anikezwe ikhemikhali. umkhiqizi ngaphambi kokugcina, ukuphatha, noma ukusebenza nanoma imaphi amakhemikhali noma izinto eziyingozi.
2.1 Nciphisa ukuthintana namakhemikhali. Gqoka izinto zokuzivikela ezifanele lapho uphatha amakhemikhali (isibonelo, izibuko zokuphepha, amagilavu, noma izingubo zokuzivikela).
2.2 Yehlisa ukuhogela kwamakhemikhali. Ungashiyi iziqukathi zamakhemikhali zivuliwe. Sebenzisa kuphela ngokungenisa umoya okwanele (isibonelo, i-fume hood).
2.3 Hlola njalo ukuvuza kwamakhemikhali noma ukuchitheka. Uma kuvuza noma ukuchitheka kwenzeka, landela izinqubo zokuhlanza zomkhiqizi njengoba kunconyiwe ku-SDS.
2.4 Bamba udoti wamakhemikhali endaweni yokushisa.
2.5 Ukuqinisekisa ukusetshenziswa kweziqukathi zemfucuza eziyisisekelo nesesibili. (Imfucuza yokuqala
isitsha sibamba imfucuza ngaleso sikhathi. Isitsha sesibili siqukethe ukuchitheka noma ukuvuza okuvela esitsheni sokuqala. Zombili iziqukathi kufanele zihambisane nemfucumfucu futhi zihlangabezane nezidingo zikahulumeni, zombuso, nezendawo zokugcinwa kweziqukathi.).
2.6 Ngemva kokuthulula isitsha sikadoti, sivale ngesivalo osinikeziwe.
2.7 Faka uphawu (ngokuhlaziya uma kunesidingo) udoti okhiqizwe yizinto ezithile ezisetshenziswayo, ama-reagents, nama-substrates asetshenziswa elabhorethri yakho.
2.8 Qinisekisa ukuthi imfucuza igcinwa, iyadluliswa, iyathuthwa, futhi ilahlwa ngokuvumelana nayo yonke imithetho yendawo, yesifunda/yesifundazwe kanye/noma kazwelonke.
2.9 Izinto ezikhipha imisebe noma ze-biohazardous zingadinga ukuphathwa okukhethekile, futhi kungasebenza imikhawulo yokulahlwa.
3.Biological ingozi ukuphepha
I-Biohazard engenzeka. Ngokuya ngamasampula asetshenziswe kuleli thuluzi, indawo engaphezulu ingase ithathwe njenge-biohazard. Sebenzisa izindlela ezifanele zokuqeda ukungcola lapho usebenza nama-biohazards.
I-BIOHAZARD. Amasampula ezinto eziphilayo njengezicubu, uketshezi lomzimba, izinto ezithathelwanayo, negazi labantu nezinye izilwane anamandla okudlulisa izifo ezithathelwanayo. Landela yonke imithetho esebenzayo yendawo, yesifunda/yesifundazwe, kanye/noma kazwelonke. Gqoka okokuvikela okufanelekile, okuhlanganisa kodwa okungakhawulelwe kulokhu: izingubo zamehlo ezivikelayo, isivikelo sobuso, izingubo/ijazi elebhu, namagilavu. Wonke umsebenzi kufanele wenziwe ezikhungweni ezihlome ngokufanelekile kusetshenziswa okokusebenza okufanele kokuphepha (isibonelo, izinto ezibambayo ezibonakalayo). Umuntu ngamunye kufanele aqeqeshwe ngokuvumelana nezimfuneko ezisebenzayo zokulawula kanye nenkampani/isikhungo ngaphambi kokusebenza ngezinto ezingaba izifo ezithathelwanayo.
Funda futhi ulandele imihlahlandlela esebenzayo kanye/noma izimfuneko zomthetho kulokhu okulandelayo:
E-US: Imihlahlandlela yoMnyango Wezempilo Nezinkonzo Zabantu wase-US eshicilelwe ku-Biosafety in Microbiological and Biomedical Laboratories etholakala ku: www.cdc.gov/biosafety.
Amazinga Okuphepha Nezempilo Emsebenzini, Amagciwane Atholakala Ngegazi (29 CFR§1910.1030), atholakala kokuthi:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
Iphrothokholi yenkampani/isikhungo sakho sohlelo lwe-Biosafety lokusebenza/ngokuphatha izinto ezingase zibe izifo ezithathelanayo. Ulwazi olwengeziwe mayelana nemihlahlandlela ye-biohazard luyatholakala ku-: www.cdc.gov.
E-EU: Hlola imihlahlandlela yendawo kanye nemithetho mayelana ne-biohazard kanye ne-biosafety precaution futhi ubhekisele kuzinqubo ezihamba phambili ezishicilelwe ku-World Health Organization (WHO) Laboratory Biosafety Manual, uhlelo lwesithathu, olutholakala ku-: www.who.int/csr/resources/publications /biosafety/WHO_CDS_CSR_LYO_200 4_11/zu/.
Imikhiqizo ehlobene
-
I-SARS-CoV-2 Antigen Assay Kit (I-Immunochromatogr...
I-SARS-CoV-2 Antigen Assay Kit (I-Immunochromatography Method) Imanuwali Yomkhiqizo 【IGAMA LOMTHETHO】SARS-CoV-2 Antigen Assay Kit （Indlela ye-Immunochromatography) 【IZINCACISO ZOKUPHAKAMA】 1 Test/Kit noveli ye-ABVEL ye-coronavirus I-COVID-19 yisifo esithathelwanayo sokuphefumula esiyingozi. Ngokuvamile abantu bayathinteka. Njengamanje, iziguli ezingenwe yi-coronavirus yenoveli yizona ezihamba phambili ...